Journal: Journal of Neurotrauma

Article Title: A Systematic Review of Cellular Transplantation Therapies for Spinal Cord Injury

doi: 10.1089/neu.2009.1177

Figure Lengend Snippet: Bone Marrow-Derived Stromal Cells – BMSC – Mesenchymal Stem Cells The stromal cells from the bone marrow are isolated and separated from the hematopoetic cell fraction of the bone marrow by their predilection to adhere to plastic. Some authors exclude the contamination by hematopoetic cells (which are CD 34 positive) by FACS. BMSCs are hence a crude mixture of stromal cells which support the growth of hematopoetic stem cells and mesenchymal stem cells. Some authors provide some additional (somewhat unspecific) markers to characterize these mesenchymal stem cells. Hence the actual stromal versus mesenchymal stem cell nature of the transplanted cells is unclear in many studies.

Article Snippet: Lee Neurop-athology 2003 Model : Adult C57BL/6 mice , 15–20 g Injury : T10 contusion, 0.25 mm at 40 ms using pneumatic impact device ▪ Adherent BMSCs from 5–8 week old C57BL/6 male mice ; 2 nd /3 rd passage; Hoechst 33342 labeled cells suspended in DMEM at 1 × 10 3 cells per μl) and injected directly into (1.5 μl) & 2 mm rostral to the lesion (1.5 μl) @ 1 wk PI • Immunosuppression : None SUBACUTE 1.

Techniques: Isolation, Injection, Labeling, Control, Transplantation Assay, Functional Assay, Modification, Saline, Passaging, IV Injection, Marker, Membrane, Migration, Produced, Western Blot, Clinical Proteomics, Magnetic Resonance Imaging, Immunohistochemistry, Expressing, Suspension, In Vitro, Cell Culture, Ex Vivo, Mouse Assay, Transgenic Assay, Transfection, Sterility, TUNEL Assay, Recombinant, Microscopy, In Vivo, Imaging, Radioactivity, H&E Stain, Staining, In Vivo Imaging, Activity Assay, Immunohistochemical staining, Infection, Light Microscopy, Disruption, Retroviral, Transduction, Enzyme-linked Immunosorbent Assay, Genetically Modified, Plasmid Preparation, Comparison

Journal: Scientific Reports

Article Title: Prognostic relevance of hemodialysis for short-term survival in patients after LVAD implantation

doi: 10.1038/s41598-018-26515-0

Figure Lengend Snippet: Patient characteristics at time of LVAD implantation.

Article Snippet: All patients received a continuous-flow 3 rd generation LVAD (HVAD Heartware Inc., Framingham, USA) for a severe HF at our institution.

Techniques: Functional Assay

Journal: Scientific Reports

Article Title: Prognostic relevance of hemodialysis for short-term survival in patients after LVAD implantation

doi: 10.1038/s41598-018-26515-0

Figure Lengend Snippet: Cause of death 30 days after LVAD (total n = 14).

Article Snippet: All patients received a continuous-flow 3 rd generation LVAD (HVAD Heartware Inc., Framingham, USA) for a severe HF at our institution.

Techniques:

Journal: Scientific Reports

Article Title: Prognostic relevance of hemodialysis for short-term survival in patients after LVAD implantation

doi: 10.1038/s41598-018-26515-0

Figure Lengend Snippet: Retention parameters at LVAD implantation and after 30 days. ( A ) Creatinine. ( B ) BUN. ( C ) eGFR *** P < 0.01. MDRD (Modification of Diet in Renal Disease).

Article Snippet: All patients received a continuous-flow 3 rd generation LVAD (HVAD Heartware Inc., Framingham, USA) for a severe HF at our institution.

Techniques: Modification

Journal: Scientific Reports

Article Title: Prognostic relevance of hemodialysis for short-term survival in patients after LVAD implantation

doi: 10.1038/s41598-018-26515-0

Figure Lengend Snippet: ( A ) Kaplan Meier survival curve 30 days after LVAD implantation. Log rank P = 0.004. ( B ) Kaplan Meier survival curve 30 days after LVAD implantation. Log rank P = 0.003.

Article Snippet: All patients received a continuous-flow 3 rd generation LVAD (HVAD Heartware Inc., Framingham, USA) for a severe HF at our institution.

Techniques:

Journal: bioRxiv

Article Title: Impaired oxidative phosphorylation drives primary tumor escape and metastasis

doi: 10.1101/2025.01.08.631936

Figure Lengend Snippet: (A) Western blot analysis of the yeast complex I protein NDI1 stable expression in wild-type RENCA cells. Western blot analysis of ECAD protein expression in 40 µg of protein lysed from EV and NDI1 expressing RENCA cells. (B) Abdominal MRI demonstrating a peri-renal lymph node metastasis in a 70-year-old male HLRCC patient. Mitochondria imaged using Transmission Electron Microscopy (TEM) in (C) normal adjacent kidney and (D) tumor from the HLRCC patient in (B). Scale bars are 0.5 µm. (E) Tumor cell culture from patient in (B), designated as the continuous tumor cell line UOK365. (F) Seahorse flux analyses of FH -/- UOK262 and UOK365 tumor cells and FH +/+ control cells. (G) GSEA Hallmark analysis of bulk-RNA sequencing of 17 primary HLRCC tumors, 12 HLRCC metastatic tumors, 6 HLRCC normal adjacent kidneys, and 12 normal kidneys from donors. While (H) oxidative phosphorylation was enriched in normal kidney compared with HLRCC tumor, (I) glycolysis and (J) EMT were enriched in tumors compared with normal kidneys.

Article Snippet: NDUFA4L2 and NDI1 3 rd -generation lentiviral expression vectors were designed with a puromycin resistance cassette and purchased from Vectorbuilder Inc. HEK293T cells were used to package the vectors into lentiviruses.

Techniques: Western Blot, Expressing, Transmission Assay, Electron Microscopy, Cell Culture, Control, RNA Sequencing Assay